| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Deoliveira, Mariana K (13)
|
MDEST |
30.31 |
30.18 |
-0.13 |
| 2 |
Brissett, Gabrielle S (14)
|
MDEST |
32.35 |
32.67 |
0.32 |
| 3 |
Saxton, Noelle E (14)
|
MDEST |
34.12 |
33.07 |
-1.05 |
| 4 |
An, Alicia D (13)
|
MDEST |
34.63 |
33.13 |
-1.50 |
| 5 |
Blaha, Sienna K (14)
|
MDEST |
34.33 |
33.28 |
-1.05 |
| 6 |
Chesnokov, Alexandra (13)
|
MDEST |
33.55 |
33.69 |
0.14 |
| 7 |
Donahue, Alexa Q (13)
|
MDMAS |
36.55 |
33.90 |
-2.65 |
| 8 |
Lee, Chaedence C (13)
|
MDEST |
35.63 |
34.01 |
-1.62 |
| 9 |
Chew, Sophia M (14)
|
MDEST |
37.26 |
35.22 |
-2.04 |
| 10 |
Karanikolis, Eleni L (14)
|
MDEST |
38.16 |
35.66 |
-2.50 |
| 11 |
Williams, Delaney L (13)
|
MDEST |
44.12 |
36.20 |
-7.92 |
| 12 |
Li, Belle (13)
|
MDEST |
47.93 |
38.35 |
-9.58 |
| 13 |
Adams, Kaylee O (14)
|
MDMAS |
34.55 |
38.52 |
3.97 |
| 14 |
Bartnik, Aubrey L (14)
|
MDSPY |
44.19 |
39.47 |
-4.72 |
| 15 |
Britt, Clara N (13)
|
MDMAS |
43.99 |
41.07 |
-2.92 |
| 16 |
Heffernan, Rowan G (14)
|
MDMAS |
34.92 |
41.22 |
6.30 |
| 17 |
Butler, Kyleigh G (13)
|
MDEST |
40.46 |
41.95 |
1.49 |
| 18 |
Bley, Taylor R (14)
|
MDNBAC |
50.84 |
42.55 |
-8.29 |
| 19 |
Roberts, Kamryn G (14)
|
MDMAS |
55.54 |
42.98 |
-12.56 |
| 20 |
Hudson, London K (13)
|
MDEST |
59.84 |
45.24 |
-14.60 |
| 21 |
Gokul, Ishanvi (13)
|
MDMAS |
|
48.90 |
- |
| 22 |
Daniels, Peighton O (13)
|
MDMAS |
|
50.00 |
- |